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Antibody Engineering & Recombinant Production Services
Antibody Customization Fueling Research Success
Bio X Cell has produced and purified antibodies from hybridomas for over 25 years while continuously studying and developing innovative ways to provide antibodies to the research community. We have applied our cell culture expertise to diverse cell types, including HEK293 and CHO, and are dedicated to stringent purification protocols to serve researchers’ recombinant antibody needs.
We can customize your specific antibody by choosing the ideal species and isotype for your application or to reduce immunogenicity. In fact, no matter your project’s needs, you likely won’t have to start from scratch, as we can customize any antibody in our extensive catalog.
Options include measures such as adding Fc region mutations to eliminate or enhance Fc receptor binding. Once your expression plasmid has been engineered, our experts convert your DNA into the antibody needed for your studies.
Discuss Your Program Needs
All antibodies available in milligram to gram scale
Supporting DNA design and synthesis through antibody purification
All products optimized for in vivo and in vitro organoid and organ on chip studies
Custom-tailored version of catalog antibodies
HEK293 or CHO cells cultured in serum-free media
Up to 20L transfected in a single production run
Supporting your project from pilot study to IND
Antibody Production Project Workflow
| Phase | Description | Included | Optional Add-Ons |
|---|---|---|---|
| Antibody Engineering & Design | Bio X Cell’s in-house experts design the antibody to your desired specs |  |  |
| DNA synthesis to support transfection volumes of 1L or less | | |
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| Maxi, mega, and gigaprep options to produce DNA to support large-scale expression | | |
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| Pilot | Assessment of antibody expression levels to determine scale-up volume | | |
| Purify small amount of antibody to ship for testing | | |
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| Transfection | Transfection of one or more plasmids into HEK293 or CHO cells in volumes from 100ml to 20L | | |
| Addition of enhancers to increase yield | | |
|
| Concentration of cell supernatant | | |
|
| Purification | Purification of supernatant over Protein A or G | | |
| Buffer exchange to PBS (preservative & stabilizer free) | | |
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| Sterile filtering | | |
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| Testing to confirm ≤1 EU/mg endotoxin levels; additional testing and antibody purification services available | | |
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| Vialing & QC | Sterile vialing of finished product | | |
| Internal quality assessment | | |
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| Certificate of Analysis (CoA) | | |
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| Analytical Size Exclusion Chromatography | | |
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| Murine pathogen screening service | | |
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| Shipping on cold packs in environmentally friendly packaging | | |
Explore Our Comprehensive Custom Antibody Solutions
InVivoMAb™ Antibody Production from Hybridoma & Stable Cell Lines
Antibody Conjugation Services
Antibody Quality Control & Testing Services
Consult With Bio X Cell to Enable Your Next Breakthrough Discovery
Whether you need antibody customization or high-volume production, Bio X Cell is committed to advancing your therapeutic innovations.
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