Large Scale Transient Recombinant Antibody Expression Services
At Bio X Cell, we have been producing and purifying antibodies from hybridomas for over 25 years, while continuously studying and exploring the innovative new ways to provide antibodies to the research community. We have applied our novel fermentation techniques to new cell types and enhanced our purification protocols to break into the recombinant antibody field.
You now have the flexibility to design your dream antibody, one with the ideal species and isotype, that has mutations to eliminate Fc receptor binding, reduce immunogenicity, or enhance antigen binding. Once your expression plasmid has been engineered, enlist our experts to convert your DNA into the antibody needed for your studies. From milligrams to grams, ready for in vivo or in vitro experimentation, Bio X Cell can support each stage of your research.
Our mammalian expression system utilizes HEK293 cells cultured in serum free media. We can transfect up to 20L in a single production run. The antibodies are purified from the clarified supernatant and have endotoxin levels of less than 2 EU per milligram. A technical project manager will help design and guide you through each phase of your project.
Explore Bio X Cellās new line of recombinant antibodies, our RecombiMAbsā¢, which are engineered versions of the original antibodies. If you need one of our catalog antibodies modified to have a different species, isotype, or a custom mutation, we can help. We will sequence the antibody variable regions, create the custom expression plasmid, and produce and purify a custom recombinant version of the antibody for you.
Project Workflow
| Phase | |||
|---|---|---|---|
| 1. Pilot | Assessment of antibody expression levels to determine scale up volume. | ||
| Purify small amount of antibody to ship for testing. | |||
| 2. Transfection | Transfection of one or more plasmids into HEK293 cells in volumes from 500ml to 20L. | ||
| Addition of enhancers to increase yield. | |||
| Concentration of cell supernatant. | |||
| 3. Purification | Purification of supernatant over Protein A or G. | ||
| Buffer exchange to PBS (preservative & stabilizer free). | |||
| Sterile filtering. | |||
| Testing to confirm <2EU/mg endotoxin (Lower endotoxin levels available upon request). | |||
| 4. Vialing & QC | Sterile vialing of finished product. | ||
| Internal quality assessment. | |||
| Certificate of analysis (CoA) | |||
| Murine pathogen screening service. | |||
| 5. Shipping | Shipping on cold packs in environmentally-friendly packaging. |
"I am totally satisfied with the service Bio X Cell has given us. When anyone asks me for CTLA-4 antibodies, I always refer them to you. I look forward to another 20 years."
- James P. Allison
Co-Recipient of the 2018 Nobel Prize in Physiology or Medicine
Customer Testimonials
Donāt see the antibody you need in our catalog, or have hybridoma cells producing your own unique antibody?
Bio X Cell can grow any hybridoma cell line and purify the antibodies via our contract antibody production services. Our custom produced antibodies are formulated the same way as our catalog products; with ultra-low endotoxin levels, high-purity, and provided in PBS with no preservatives or stabilizers added.Contact contractservices@uat.bioxcell.com to tell us more about your antibody needs.