InVivoMAb anti-human/mouse GRP78
Catalog #BE0396
Clone:
C38
Product Details
The C38 monoclonal antibody reacts with human and mouse Glucose Regulated Protein 78 (GRP78) also known as BiP or HSPA5. GRP78 is a stress inducible, pro-survival, endoplasmic reticulum chaperone in the HSP70 family. Cell Surface GRP78 acts as a multifunctional receptor that affects both cell proliferation and viability. GRP78 is involved in many cellular processes, including translocating newly synthesized polypeptides across the ER membrane, facilitating the folding and assembly of proteins, targeting misfolded proteins for ER-associated degradation (ERAD), regulating calcium homeostasis, and serving as an ER stress sensor. Expression of cell surface GRP78 is associated with increased malignancy and resistance to chemotherapy and radiotherapy in various cancers, particularly prostate cancer. The C38 antibody binds to the Cāterminal domain of GRP78 and has been shown to have anti-tumoral potential in various in vitro and in vivo tumor models.Specifications
| Isotype | Mouse IgG2b, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb mouse IgG2b isotype control, unknown specificity |
| Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
| Immunogen | Full-length recombinant murine GRP78 protein |
| Reported Applications |
in vivo GRP78 blockade in vitro GRP78 blockade Western blot Flow cytometry |
| Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein A |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb mouse IgG2b isotype control, unknown specificity
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Recommended Dilution Buffer
InVivoPure pH 7.0 Dilution Buffer
