Functional Assays
Bio X Cell antibodies are specifically designed for functional assays, enabling researchers to evaluate the biological activity of antibodies in experimental systems. These assays are essential for determining whether an antibody elicits the desired response in in vitro organoid and organ-on-chip studies or in in vivo models.
Bio X Cell antibodies have been cited in nearly 30,000 peer-reviewed publications and can be applied across a wide range of functional studies, including:
- Neutralization: blocking cytokines, chemokines, or receptors
- Pathway Blocking: preventing receptor–ligand interactions
- Activation/Stimulation: triggering immune cell signaling pathways
- Cell Depletion: selectively removing specific immune cell populations
All Bio X Cell antibodies are formulated for sensitive applications, featuring ultrapure (≥95% purity) quality, low endotoxin levels (as low as ≤0.5 EU/mg), and no preservatives, stabilizers, or carrier proteins. Every lot is backed by a Certificate of Analysis (CoA) to ensure reproducibility and transparency.
Functional Assay Applications and Example Targets
| Assay Type | Purpose | Example Antibody Targets |
|---|---|---|
| Neutralization | Block cytokine or chemokine activity | TNF-α, IL-6, IFN-γ, IL-10, TGF-β |
| Pathway Blocking | Prevent receptor–ligand signaling | PD-1 / PD-L1, CTLA-4, CD40L, CD28 |
| Activation / Stimulation | Trigger immune cell responses and costimulatory pathways | CD3, CD28, CD40, OX40, 4-1BB |
| Cell Depletion | Selectively remove immune cell populations | CD4 (T helper cells), CD8 (cytotoxic T cells), CD20 (B cells), NK1.1 (NK cells) |
| Engagement / Targeting | Explore binding interactions with antigens/molecules | LAG-3, TIGIT, ICOS, chemokine receptors (e.g., CCR5) |
