InVivoMAb anti-mouse RGMb
Catalog #BE0400
Clone:
307.9D1
Reactivities:
Mouse
Product Details
The 307.9D1 monoclonal antibody reacts with mouse Repulsive Guidance Molecule b (RGMb), a member of the RGM family which consists of RGMa, RGMb, and RGMc/hemojuvelin. RGMs are GPI-anchored membrane proteins that bind bone morphogenetic proteins (BMPs) and neogenin. BMPs have diverse roles in many processes, including cell proliferation, differentiation, and apoptosis. RGMb has a well-elucidated role in neural development, with RGMb knock-out mice dying 2-3 weeks after birth. RGMb is also expressed by macrophages and can regulates IL-6 expression. Recently, it has been shown that PD-L2 also binds to RGMb and that blocking the RGMbāPD-L2 interaction impairs the development of respiratory tolerance but blocking the PD-1āPD-L2 interaction does not. The 307.9D1 antibody has been shown to block the binding of RGMb to PD-L2 and BMP-2/4.Specifications
| Isotype | Rat IgG2a, Īŗ |
|---|---|
| Recommended Isotype Control(s) | InVivoMAb rat IgG2a isotype control, anti-trinitrophenol |
| Recommended Dilution Buffer | InVivoPure pH 6.0T Dilution Buffer |
| Immunogen | Recombinant murine RGMb |
| Reported Applications |
in vivo RGMb blockade ELISA Flow cytometry |
| Formulation |
PBS, pH 6.0 Contains no stabilizers or preservatives |
| Endotoxin |
<2EU/mg (<0.002EU/Ī¼g) Determined by LAL gel clotting assay |
| Sterility | 0.2 Āµm filtration |
| Production | Purified from tissue culture supernatant in an animal free facility |
| Purification | Protein G |
| Molecular Weight | 150 kDa |
| Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |
Recommended Products
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Recommended Isotype Control(s)
InVivoMAb rat IgG2a isotype control, anti-trinitrophenol
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Recommended Dilution Buffer
InVivoPure pH 6.0T Dilution Buffer