RecombiMAb anti-mouse Ly6G
Catalog #CP129
Clone:
1A8-CP129
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG2a)
(switched from rat IgG2a)
Product Details
The 1A8-CP129 monoclonal antibody is a chimeric version of the original 1A8 antibody. The variable domain sequences are identical to the original 1A8 but the constant region sequences have been switched from rat IgG2a to mouse IgG2a. The 1A8-CP129 antibody contains no Fc mutations just as the original rat IgG2a antibody does not. The 1A8-CP129 monoclonal antibody reacts with mouse Ly6G. Ly6G is a 21-25 kDa member of the Ly-6 superfamily of GPI-anchored cell surface proteins with roles in cell signaling and cell adhesion. Ly6G is expressed differentially during development by cells in the myeloid lineage including monocytes, macrophages, granulocytes, and neutrophils. Monocytes typically express Ly6G transiently during development while mature granulocytes and peripheral neutrophils retain expression making Ly6G a good cell surface marker for these populations. Unlike the RB6-8C5 antibody, the 1A8-CP129 antibody reacts specifically with mouse Ly6G with no reported cross reactivity with Ly6C.Specifications
Isotype | Mouse IgG2a, Īŗ |
---|---|
Recommended Isotype Control(s) | InVivoPlus mouse IgG2a isotype control, unknown specificity |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Immunogen | EL4J cells transfected with Ly6G |
Reported Applications |
in vivo neutrophil depletion* in vivo MDSC depletion* Immunofluorescence* Immunohistochemistry (paraffin)* Immunohistochemistry (frozen)* Flow cytometry* *Reported for the original rat IgG2a 1A8 antibody |
Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
Sterility | 0.2 Āµm filtration |
Production | Purified from CHO cell supernatant in an animal free facility |
Purification | Protein G |
Molecular Weight | 150 kDa |
Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |