RecombiMAb anti-mouse GITR (D265A)

Catalog #CP027
Clone:
DTA-1-CP027
Reactivities:
Mouse

$500.00

$500.00

Choose an Option...
  • 1 mg - $500.00
  • Custom Amount (Quotes Only)
In stock
Only %1 left
Isotype:
Mouse IgG1
(switched from rat IgG2b)

Product Details

The DTA-1-CP027 monoclonal antibody is a chimeric version of the original DTA-1 antibody. The variable domain sequences are identical to the original DTA-1 but the constant region sequences have been switched from rat IgG2b to mouse IgG1. The DTA-1-CP027 antibody also contains a D265A mutation in the Fc fragment rendering it unable to bind to endogenous FcĪ³ receptors. The DTA-1-CP027 antibody reacts with mouse GITR (glucocorticoid-induced TNFR-related gene), a 66-70 kDa co-stimulatory immune checkpoint molecule belonging to the Tumor Necrosis Factor superfamily (TNFRSF18). GITR is expressed at low levels on resting T lymphocytes and at high levels on regulatory T cells. GITR is upregulated on activated T cells where it provides co-stimulation. GITR ligand (GITRL) is found on B cells, macrophages, dendritic and endothelial cells, and is implicated in regulating both innate and adaptive immune responses. GITR is also thought to play a key role in dominant immunological self-tolerance maintained by regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T cell activation and programmed cell death. The DTA-1 antibody is an agonistic antibody that is commonly used to induce GITR signaling in vivo.

Specifications

Isotype Mouse IgG1, Īŗ
Recommended Isotype Control(s) RecombiMAb mouse IgG1 (D265A) isotype control, anti-hen egg lysozyme
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Mutations D265A
Immunogen Mouse CD25+Ā CD4+Ā T cells
Reported Applications in vivo GITR stimulation*
*Reported for the original rat IgG2b DTA-1 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/Ī¼g)
Determined by LAL gel clotting assay
Sterility 0.2 Āµm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze.