RecombiMAb anti-mouse GITR (LALA-PG)
Catalog #CP029
Clone:
DTA-1-CP029
Reactivities:
Mouse
Isotype:
Mouse IgG2a
(switched from rat IgG2b)
(switched from rat IgG2b)
Product Details
The DTA-1-CP029 monoclonal antibody is a chimeric version of the original DTA-1 antibody. The variable domain sequences are identical to the original DTA-1 but the constant region sequences have been switched from rat IgG2b to mouse IgG2a. The DTA-1-CP029 antibody also contains a LALA-PG mutation in the Fc fragment rendering it unable to bind to endogenous FcĪ³ receptors. The DTA-1-CP029M antibody reacts with mouse GITR (glucocorticoid-induced TNFR-related gene), a 66-70 kDa co-stimulatory immune checkpoint molecule belonging to the Tumor Necrosis Factor superfamily (TNFRSF18). GITR is expressed at low levels on resting T lymphocytes and at high levels on regulatory T cells. GITR is upregulated on activated T cells where it provides co-stimulation. GITR ligand (GITRL) is found on B cells, macrophages, dendritic and endothelial cells, and is implicated in regulating both innate and adaptive immune responses. GITR is also thought to play a key role in dominant immunological self-tolerance maintained by regulatory T cells. Knockout studies in mice also suggest the role of this receptor is in the regulation of CD3-driven T cell activation and programmed cell death. The DTA-1 antibody is an agonistic antibody that is commonly used to induce GITR signaling in vivo.Specifications
Isotype | Mouse IgG2a, Īŗ |
---|---|
Recommended Isotype Control(s) | RecombiMAb mouse IgG2a (LALA-PG) isotype control, anti-hen egg lysozyme |
Recommended Dilution Buffer | InVivoPure pH 7.0 Dilution Buffer |
Mutations | LALA-PG |
Immunogen | Mouse CD25+Ā CD4+Ā T cells |
Reported Applications |
in vivo GITR stimulation* *Reported for the original rat IgG2b DTA-1 antibody |
Formulation |
PBS, pH 7.0 Contains no stabilizers or preservatives |
Endotoxin |
<1EU/mg (<0.001EU/Ī¼g) Determined by LAL gel clotting assay |
Sterility | 0.2 Āµm filtration |
Production | Purified from HEK293 cell supernatant in an animal free facility |
Purification | Protein G |
Molecular Weight | 150 kDa |
Storage | The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze. |