RecombiMAb anti-mouse CD28

Catalog #CP042
Clone:
D665-CP042
Reactivities:
Mouse

$500.00

$500.00

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  • 1 mg - $500.00
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In stock
Only %1 left
Isotype:
Mouse IgG2a
(switched from mouse IgG1)

Product Details

The D665-CP042 monoclonal antibody is a chimeric version of the original D665 antibody. The variable domain sequences are identical to the original D665 but the constant region sequences have been switched from mouse IgG1 to mouse IgG2a. The D665-CP042 antibody contains no Fc mutations just as the original mouse IgG1 antibody does not. The D665-CP042 monoclonal antibody reacts with mouse CD28, a 45 kDa costimulatory receptor and a member of the Ig superfamily. CD28 is expressed by thymocytes, most peripheral T cells, and NK cells. CD28 is a receptor for CD80 (B7-1) and CD86 (B7-2). Signaling through CD28 induces IL-2 and IL-2 receptor expression and T cell proliferation. The D665 antibody is a CD28 superagonist and is most commonly used to induce the expansion of Treg cells in vivo in various mouse models of disease.

Specifications

Isotype Mouse IgG2a,Ā Īŗ
Recommended Isotype Control(s) InVivoPlus mouse IgG2a isotype control, unknown specificity
Recommended Dilution Buffer InVivoPure pH 7.0 Dilution Buffer
Immunogen A20 cells expressing mouse CD28 and a recombinant mouse CD28-Ig fusion protein
Reported Applications in vivo T cell stimulation/activation*
in vitro T cell stimulation/activation*
*Reported for the original mouse IgG1 D665 antibody
Formulation PBS, pH 7.0
Contains no stabilizers or preservatives
Endotoxin <1EU/mg (<0.001EU/Ī¼g)
Determined by LAL gel clotting assay
Sterility 0.2 Āµm filtration
Production Purified from HEK293 cell supernatant in an animal free facility
Purification Protein G
Molecular Weight 150 kDa
Storage The antibody solution should be stored at the stock concentration at 4Ā°C. Do not freeze.

Additional Formats